Journal of Clinical and Investigative Dermatology
Research Article
Evidence for Microvesicle Particles in UVB-Mediated IL-8 Generation in Keratinocytes
Bhadri S1, Thapa P1,Chen Y1, Rapp CM1 and Travers JB1-3*
1Departments of Pharmacology and Toxicology, Boonshoft School of
Medicine at Wright State University, USA
2Department of Dermatology, Boonshoft School of Medicine at Wright
State University, USA
3Department of Dermatology, The Dayton V.A. Medical Center, USA
*Address for Correspondence: Travers JB, Department of Pharmacology and Toxicology,
Boonshoft School of Medicine at Wright State University 3640 Col
Glenn Hwy Dayton, OH 45435, USA, Email: Jeffrey.travers@wright.
edu
Submission: 30 November, 2021;
Accepted: 26 December, 2021;
Published: 30 December, 2021
Copyright: © 2021 Bhadri S, et al. This is an open access article
distributed under the Creative Commons Attri-bution License,
which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
Abstract
Recent studies have implicated bioactive microvesicle particles
(MVP) in the keratinocyte response to many environmental stressors,
in partricular ultraviolet B radiation (UVB). The generation of MVP in
response to UVB involves the Platelet-activating factor receptor (PAFR)
and the enzyme acid sphingomyelinase (aSMase). As UVB generates
some cytokines such as interleukin-8 (IL-8) in a PAFR-dependent manner,
one question is if the production and release of IL-8 and MVP could
be linked. Using the human keratinocyte-derived cell line HaCaT,
the present in vitro studies indicate that pretreatment of HaCaT
keratinocytes with PAFR agonist can synergize with low fluences of UVB
to generate high levels of MVP as well as IL-8 protein. Treatment of
cells with an aSMase pharmacologic inhibitor blocked both processes.
These studies indicate the possibility that MVP could be involved in
pathologic processes involving UVB-generated production of proinflammatory
cytokines such as IL-8.