Journal of Analytical & Molecular Techniques

Research Article

Parboiled Preservation of Odonata Nymphs for DNA Related Research

Sutherland LN1*, Fomekong-Lontchi J1,5*, Lupiyaningdyah P1,4, Tennessen KJ3, Carter P1 and Bybee SM1,2

1Department of Biology, Brigham Young University, 4102 LSB, Provo, UT 84602, USA
2Bean Life Science Museum, Brigham Young University, Provo, UT 84602, USA
3Florida State Collection of Arthropods, Gainesville, Florida, USA
4Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia
5Institute of Medical Research and Medicinal Plants Studies (IMPM), Centre of Medical Research, P.O Box: 6163 Yaoundé, Cameroon
*Address for Correspondence:Sutherland LN, Department of Biology, Brigham Young University, 4102 LSB, Provo, UT 84602, USA E-mail Id: lns25@byu.edu
Submission:09 December, 2024 Accepted:15 January, 2025 Published:20 January, 2025
Copyright: ©2025 Sutherland LN, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords:Dragonfly; Damselfly; Nymphs; DNA; Parboil

Abstract

Odonate nymph preservation is less standardized and more prone to long-term preservation issues than adults.Two preemptive measures that can be taken to help prevent the high level of decay often seen in older nymph specimens are 1. Ethanol injection 2. Parboiling. Parboiling offers the long-term advantage of better retaining the morphology, which is a great advantage for future taxonomic projects. Here we found that parboiling is as good for preserving DNA as ethanol injection.