A cross sectional study was conducted on 384 randomly selected sheep in and around Jimma town, Oromia Regional State, Southwest Ethiopia to assess the prevalence of ovine mange mites by laboratory examination using skin scrappy. The results of the microscopical examination of the skin scraping revealed that 37 of the sheep were infested with mites with an overall prevalence of 9.6%. In this study, three genus of mange mites were recorded that parasitized sheep; namely, Sarcoptes, Psoroptes and Demodex with prevalence of 3.38%, 5.2% and 1.04% respectively, which were found on the back, shoulder, tail, ear, face, ventral abdomen regions of the animals’ body. The prevalence of the infestation was highest in sheep younger than two years (12.4%) and the lowest in sheep with age older than two years (7.6%). The prevalence of mange mites in male sheep was 9.1% and 10.2% in females. There were no statistically significant difference (p>0.05) in the prevalence of mange mite infestation between the different age and sex groups. The difference in the prevalence of mange mites infestation in body condition score and management practices was statistically significant (χ2=24.613, p=0.000), (χ2=13.627, p=0.01, respectively). The dominant lesions of mange mites were formations of nodules and crusts and also loss of hair and ragged wool. Therefore, there should be immediate attention and control interventions against the disease to cut the losses that hamper sheep production and productivity in the study area.