L-tryptophanis an essential amino acid, essential for normal growth of child and adults, protein synthesis, precursor of serotonin, melatonin, and niacin. The research work was designed to investigate the impact of the Trivedi Effect - Consciousness Energy Treatment on the structural properties and the isotopic abundance ratio of L-tryptophan using liquid chromatography – mass spectrometry analytical technique. The L-tryptophan sample was divided into Control and the Biofield Energy Treated tryptophan. The treated tryptophan sample received Biofield Energy Treatment (the Trivedi Effect ®) remotely for ~3 minutes by Mr. Mahendra Kumar Trivedi, who was located in the USA, while the test samples were located in the research laboratory in India. The mass spectra of both the tryptophan samples at retention time 2 minutes exhibited the molecular ion mass peak adduct with hydrogen ion at m/z 205 (C₁₁H₁₃N₂O₂⁺® ) along with low molecular fragmented mass peaks at m/z 188 and 102 for C₁₁H₁₂N₂O₂₊ and C₈H₆⁺, respectively. The isotopic abundance ratio of P_M+1/P_M (²H/¹ or¹³C/¹²C or ¹⁵N/¹⁴N or ¹⁷O/¹⁶O) in the Treated L-tryptophan was significantly increased by 21.89%compared with the Control sample. Therefore, ¹³C, ²H⁵N, and ¹⁷O contributions from C₁₁H₁₃N₂O to m/z 206.08 in the Treated tryptophan was increased significantly compared to the Control sample. The change in the isotopic abundance might be due to the modification in nuclei possibly through the interference of neutrino particles with the help of the Trivedi Effect 2+-Consciousness Energy Treatment. The increased isotopic abundance ratio of the Treated tryptophan may increase the intra-atomic bond strength, increase its stability, and shelf life. The Biofield Energy Treated tryptophan might have increased the stability and shelf-life compared to the Control sample. The Treated tryptophan would be more stable in the nutraceutical, and pharmaceutical formulations, which would be advantageous for the prevention and treatment of pellagra, depression, kynurenine. It could also maintain the normal label of tryptophan and avoid increase of its metabolite, lower the neurotoxin and a metabotoxin behavior, glutaric aciduria type I disorder, eosinophilia-myalgia syndrome, etc. The Treated L-tryptophan would be advantageous for the improvement of yield, productivity, and quality of crops and other plants.