Xylene and xylene substitutes are commonly used as clearing agents when processing tissue samples during Mohs micrographic surgery (MMS). However, there are several concerns with these solvents. Xylene is expensive, malodorous, and can be toxic depending on exposure levels. While xylene substitutes are less toxic, some of these are more expensive than xylene itself or do not biodegrade easily and require specific waste disposal. Here, we discuss an alternative using Ultraviolet (UV) Clear PermaSlip Mounting Medium and a UV light. This technique is advantageous in that it is non-toxic, has no odor, and does not have the significant costs of purchase and disposal that xylene and xylene substitute typically have. However, we found this technique can add up to 2 minutes to the process, and more importantly, may reduce the quality of the slide. Upon slide review with this method, we found more intracellular lacunae artifact with keratinocytes in the epidermis compared to the same tissue on consecutive cuts where traditional xylene substitute was used. Future studies could look at ways to minimize lacunae with this method. Ultimately, we must weigh the advantages and disadvantages of using a UV mounting medium and UV light and utilize our different options to provide the best quality patient care