A cross-sectional study was conducted from November 2017 to May 2018 on selected dairy farms in Hawassa town to isolate and assess the in-vitro antibiotic biogram of Salmonella from lactating dairy cows, personnel’s and equipments at farms. A total of 216 samples were collected from selected dairy farms. All samples were processed bacteriologically following standard procedures outlined by ISO 6579: 2002. The overall prevalence of Salmonella was 12.9% (28/216) of the total samples. Out of total, 64.3% (18/28), 10.7% (3/28) and 25% (7/28) were from lactating cows, personnels’, and equipments, respectively. Based on antimicrobial susceptibility testing, all isolates were resistant at least to one or more antimicrobials tested. Accordingly, 96.4% (27/28), 82.1% (23/28) and 75.0% (21/28) isolates showed resistance for oxytetracycline, kanamycin, and nalidixic acid, respectively. Out of all the resistant isolates, 96.4% (27/28) showed multiple antibiotic resistance (resistance to two or more antibiotics) patterns. Multiple antimicrobials resistance was observed in 66.7% (18/27), 7.4% (2/27) and 25.9% (7/27) from lactating cows, personnels’, and equipments, respectively. Thus, awareness creation to the public regarding the public health importance of multiple drug-resistant Salmonella species and the consumption of unpasteurized milk and milk products is important.